表达Ⅰ型耐热肠毒素的重组大肠杆菌对7日龄仔猪肠道免疫、屏障及抗氧化功能的影响

doi:10.11843/j.issn.0366-6964.2017.09.023

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (9): 1761-1768.doi: 10.11843/j.issn.0366-6964.2017.09.023

表达Ⅰ型耐热肠毒素的重组大肠杆菌对7日龄仔猪肠道免疫、屏障及抗氧化功能的影响

吕阳¹, 张林², 李雪妮¹, 赵迪¹, 易丹¹, 陈洪波¹, 吴涛^1*

1. 武汉轻工大学动物营养与饲料科学湖北省重点实验室, 武汉 430023;
2. 中国水产科学研究院长江水产研究所, 武汉 430223

收稿日期:2017-03-22 出版日期:2017-09-23 发布日期:2017-09-23
通讯作者: 吴涛(1978-),男,湖北十堰人,博士,副教授,E-mail:wtao05@163.com
作者简介:吕阳(1993-),男,内蒙古呼伦贝尔人,硕士生,主要从事营养生化与代谢调控研究,E-mail:523738285@qq.com
基金资助:
国家自然科学基金（31302089）；湖北省自然科学基金（2012FFBO4804）

Effects of Recombinant Escherichia coli Expressing Heat-stable Enterotoxin on Intestinal Immune and Barrier Function and Antioxidant Capacity of Seven Days Old Piglets

LÜ Yang¹, ZHANG Lin², LI Xue-ni¹, ZHAO Di¹, YI Dan¹, CHEN Hong-bo¹, WU Tao^1*

1. Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China;
2. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China

Received:2017-03-22 Online:2017-09-23 Published:2017-09-23
Supported by:

摘要/Abstract

摘要：

旨在研究表达Ⅰ型耐热肠毒素（STa）的重组大肠杆菌对7日龄仔猪肠道吸收、屏障、转运及抗氧化功能的影响。选取24头7日龄仔猪，分成4个处理组，分别为对照组（基础日粮）、STa组（基础日粮+2×10⁹ CFU重组菌LMG194-STa）、LMG194组（基础日粮+2×10⁹ CFU大肠杆菌LMG194）和K88组（基础日粮+2×10⁹ CFU大肠杆菌K88）。使用人工乳饲养，第5天进行攻毒，第7天屠宰取样。测量回肠免疫相关基因IFN-γ、IL-4与VNN1及回肠黏膜损伤基因Villin与MMP3的相对转录量，空肠黏膜上皮连接蛋白Occludin与Claudin-1相对表达量，以及十二指肠、回肠氧化相关酶谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）、髓过氧化物酶（MPO）的活力与氧化相关产物丙二醛（MDA）的含量。试验结果表明，与对照组相比，STa组显著降低了回肠IFN-γ和IL-4基因相对转录量，VNN1相对转录量显著上调（P<0.05）；STa组显著降低了基因Villin与MMP3相对转录量（P<0.05）及蛋白Occludin与Claudin-1相对表达量（P<0.05）；同时，STa组十二指肠、回肠GSH-Px和回肠MPO活力显著降低（P<0.05）；STa组十二指肠丙二醛（MDA）含量显著升高（P<0.05）。结果显示，表达Ⅰ型耐热肠毒素的重组大肠杆菌可导致7日龄仔猪肠道免疫、屏障及抗氧化功能下降。

Abstract:

This experiment was conducted to investigate the effects of recombinant escherichia coli expressing heat-stable enterotoxin (STa) on intestinal immune and barrier function and antioxidant capacity of 7 days old piglets. Twenty-four 7 days old piglets were allotted to four treatments:control group, STa group (2×10⁹ CFU E. coli LMG194-STa), LMG194 group (2×10⁹ CFU E. coli LMG194), and K88 group (2×10⁹ CFU E. coli K88). The pigs were fed with artificial milk and slaughtered 7 days later. The result showed that, compared with the control group, STa group significantly reduced IFN-γ and IL-4 gene transcription levels (P<0.05), and increased VNN1 transcription level in ileum (P<0.05). STa group also significantly reduced gene transcription levels of Villin and MMP3 in ileum (P<0.05) and protein expression levels of Occludin and Claudin-1 in jejunum (P<0.05). STa group significantly reduced activity of glutathione peroxidase (GSH-Px) in duodenum and ileum (P<0.05), and significantly reduced activity of myeloperoxidase (MPO) in ileum (P<0.05), and had a higher production of malondialdehyde (MDA) in duodenum (P<0.05). These results suggest that recombinant Escherichia coli expressing STa can reduce intestinal immune and barrier function and antioxidant capacity of 7 days old piglets.

中图分类号:

S855.1

吕阳, 张林, 李雪妮, 赵迪, 易丹, 陈洪波, 吴涛. 表达Ⅰ型耐热肠毒素的重组大肠杆菌对7日龄仔猪肠道免疫、屏障及抗氧化功能的影响[J]. 畜牧兽医学报, 2017, 48(9): 1761-1768.

Lü Yang, ZHANG Lin, LI Xue-ni, ZHAO Di, YI Dan, CHEN Hong-bo, WU Tao. Effects of Recombinant Escherichia coli Expressing Heat-stable Enterotoxin on Intestinal Immune and Barrier Function and Antioxidant Capacity of Seven Days Old Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(9): 1761-1768.

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表达Ⅰ型耐热肠毒素的重组大肠杆菌对7日龄仔猪肠道免疫、屏障及抗氧化功能的影响

Effects of Recombinant Escherichia coli Expressing Heat-stable Enterotoxin on Intestinal Immune and Barrier Function and Antioxidant Capacity of Seven Days Old Piglets

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